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dna clean concentrator 5 columns  (Zymo Research)


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    Structured Review

    Zymo Research dna clean concentrator 5 columns
    Dna Clean Concentrator 5 Columns, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 8077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+clean+and+concentrator+column/DNA+Clean+%26+Concentrator-5/10__1021_slash_acs__biochem__6c00443-93-40-45
    Average 99 stars, based on 8077 article reviews
    dna clean concentrator 5 columns - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Three-dimensional genome landscape of primary human cancers
    Article Snippet: .. Amplified libraries were size-selected using either PAGE gel size selection after cleanup with DNA Clean and Concentrator column (Zymo) or two-sided size selection with Ampure XP beads (0.5X followed by 0.8X). .. Libraries were quantified using qPCR, diluted to 4 nM and sequenced on an Illumina HiSeq 4000 with paired-end 75 bp reads.

    Article Title: Virus Detection by CRISPR-Cas9-Mediated Strand Displacement in a Lateral Flow Assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single-base mutations were chemically synthesized (IDT), and then amplified by PCR to label with biotin.

    Article Title: Virus detection by CRISPR-Cas9-mediated strand displacement in a lateral flow assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single base mutations were chemically synthesized (IDT), then amplified by PCR to label with biotin.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Three-dimensional genome landscape of primary human cancers
    Article Snippet: .. Amplified libraries were size-selected using either PAGE gel size selection after cleanup with DNA Clean and Concentrator column (Zymo) or two-sided size selection with Ampure XP beads (0.5X followed by 0.8X). .. Libraries were quantified using qPCR, diluted to 4 nM and sequenced on an Illumina HiSeq 4000 with paired-end 75 bp reads.

    Size Selection:

    Article Title: Three-dimensional genome landscape of primary human cancers
    Article Snippet: .. Amplified libraries were size-selected using either PAGE gel size selection after cleanup with DNA Clean and Concentrator column (Zymo) or two-sided size selection with Ampure XP beads (0.5X followed by 0.8X). .. Libraries were quantified using qPCR, diluted to 4 nM and sequenced on an Illumina HiSeq 4000 with paired-end 75 bp reads.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Virus Detection by CRISPR-Cas9-Mediated Strand Displacement in a Lateral Flow Assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single-base mutations were chemically synthesized (IDT), and then amplified by PCR to label with biotin.

    Article Title: Virus detection by CRISPR-Cas9-mediated strand displacement in a lateral flow assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single base mutations were chemically synthesized (IDT), then amplified by PCR to label with biotin.

    Purification:

    Article Title: Virus Detection by CRISPR-Cas9-Mediated Strand Displacement in a Lateral Flow Assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single-base mutations were chemically synthesized (IDT), and then amplified by PCR to label with biotin.

    Article Title: Virus detection by CRISPR-Cas9-mediated strand displacement in a lateral flow assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single base mutations were chemically synthesized (IDT), then amplified by PCR to label with biotin.

    Spectrophotometry:

    Article Title: Virus Detection by CRISPR-Cas9-Mediated Strand Displacement in a Lateral Flow Assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single-base mutations were chemically synthesized (IDT), and then amplified by PCR to label with biotin.

    Article Title: Virus detection by CRISPR-Cas9-mediated strand displacement in a lateral flow assay
    Article Snippet: .. The protocol consisted of an initial step at 25 °C for 2 min for uracil-N-glycosylation and 50 °C for 15 min for RT, then an inactivation step at 95 °C for 2 min, followed by 40 cycles of amplification at 95 °C for 15 s and 60 °C for 60 s. RT-PCR products were purified using the DNA clean and concentrator column (Zymo) and quantified in a spectrophotometer (NanoDrop, Thermo). .. Besides, E gene amplicons with different single base mutations were chemically synthesized (IDT), then amplified by PCR to label with biotin.

    other:

    Article Title: Systems and methods for improved nanopore-based analysis of nucleic acids
    Article Snippet: A mixture of (a) 48 μL of 100 nM ΦX174 DNA (b) 6 μL of 10×T4 ligase buffer (New England Biolabs) (c) 2 μL of 12.5 μM threading adapters (to give 5:1 ratio of adapters to target sticky ends) (d) 2 μL of 12.5 μM loading adapters (to give 5:1 ratio of adapters to target sticky ends) (e) 1 μL of molecular biology grade water (f) 1 μL of T4 DNA ligase at 4 0 0 Units μL ( New Englad Biolabs ) was incubated at 16° C. for 60 minutes, then heat inactivated by heating to 65° C. for 10 minutes.



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